并列题名: | Diversity of bacteria affects growth, nutrition, immunity of honey bee and quality of honey in process of collecting nectar.In recent years,research was paid attention to symbiont in gut of honey bee and microbe of honey stomach. In order to explore bacterial diversity of nectar,honey stomach and honey, changes of amylase in process of bee collecting honey, dominant bacteria effecting amylase value and bacteriostasis of dominant bacteria, we analyzed 16S rDNA sequence and identified isolates and ascertained bacterial property of species and genus, based on traditional culture technology and biochemical test. We simulated bacterial changes from nectar to honey stomach and obtained the amount of bacterial amylase and degree of influence. We found that dominant bacteria secretes amylase in honey stomach. We explored the bacteriostasis of dominant bacteria as probiotic in the honey stomach, in order to provide a theoretical basis for evaluation and control of bee products quality and probiotic application. eng |